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Related Experiment Videos

The postcoital test: a quantitative method

M C Doody1, M C Good

  • 1Department of Obstetrics and Gynecology, University of Tennessee Medical Center, Knoxville.

Journal of Andrology
|March 1, 1993
PubMed
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This study introduces a modified postcoital test (PCT) using microspheres to standardize cervical mucus volume. This method improves the accuracy of sperm concentration and motility assessments, reducing variability in fertility testing.

Area of Science:

  • Reproductive Biology
  • Clinical Diagnostics
  • Microscopy Techniques

Background:

  • The postcoital test (PCT) is crucial for evaluating sperm-mucus interaction in fertility assessments.
  • Variability in microscopic analysis, particularly sperm concentration and motility, limits the PCT's diagnostic accuracy.
  • The slide-to-coverslip distance in PCT samples is a significant, uncontrolled variable.

Purpose of the Study:

  • To introduce a minor modification to the postcoital test (PCT) for volumetric inspection of cervical mucus.
  • To standardize the slide-to-coverslip distance using microspheres for accurate sperm concentration calculation.
  • To assess the impact of this modification on sperm density and motility variability.

Main Methods:

  • A minor modification of the postcoital test (PCT) was developed.

Related Experiment Videos

  • Homogeneously sized plastic microspheres (40.3-microns) were used as spacers on cervical mucus samples.
  • Microscopic analysis of sperm concentration and motility was performed with and without microspheres in 20 patients.

    • Main Results:

    • The microspheres allowed for volumetric inspection of cervical mucus, enabling calculation of actual sperm concentrations.
    • Variability in sperm density and motility measurements was significantly decreased by the application of microspheres.
    • The microspheres appear to control the slide-to-coverslip distance, a presumed source of PCT variability.

    Conclusions:

    • This modified PCT with microsphere spacers offers a more accurate and reproducible method for assessing sperm concentration and motility.
    • The standardization of sample volume enhances the diagnostic value of the postcoital test in fertility evaluations.
    • This technique addresses a key source of variability in microscopic semen analysis.