Related Experiment Videos
Variability over time of complement activation induced by air bubbles in human and rabbit sera
K Bergh1, A Hjelde, O J Iversen
1Department of Biomedical Engineering, University of Trondheim, Norway.
Journal of Applied Physiology (Bethesda, Md. : 1985)
|April 1, 1993
Summary
Air bubbles activate complement, generating anaphylatoxin des-Arg-C5a in serum. Individual complement sensitivity to air bubbles varies significantly over time, making single measurements unreliable.
Area of Science:
- Immunology
- Biochemistry
Background:
- The complement system is crucial for innate immunity.
- Air bubbles can potentially trigger immune responses.
Purpose of the Study:
- To investigate complement activation by air bubbles.
- To measure anaphylatoxin des-Arg-C5a generation.
- To assess individual variability in complement activation.
Main Methods:
- Quantification of des-Arg-C5a using sandwich enzyme-linked immunosorbent assays.
- Continuous introduction of air bubbles into rabbit and human sera.
- Measurement of des-Arg-C5a generation over extended periods.
Main Results:
- Air bubbles significantly increased des-Arg-C5a levels compared to controls.
- A dose-dependent relationship was observed between air bubble flow and des-Arg-C5a generation.
- Pronounced intraindividual variability in des-Arg-C5a levels was detected over time.
Conclusions:
- Complement activation by air bubbles is variable within individuals.
- Single-point analysis is insufficient to characterize an individual's complement system sensitivity to air bubbles.