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Isolation and characterization of a trisialyllactosylceramide, GT3, containing an O-acetylated sialic acid in cod
1Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Gerontology.
Journal of Biochemistry
|April 1, 1993
Abstract:
An O-acetylated ganglioside that generated a trisialyllactosylceramide or GT3 by base treatment was found for the first time in cod fish brain. This ganglioside was isolated by high-performance liquid chromatography, and characterized by fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance spectroscopy in addition to chemical analysis. The structure was identified as a modified GT3 in which the external sialic acid is O-acetylated at the C-9 position. The chemical structure is as follows: II3(9-O-Ac-NeuAc2-8NeuAc2-8NeuAc2-3)Lac Cer.