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LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
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LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

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Freeze-thaw studies of a model protein, lactate dehydrogenase, in the presence of cryoprotectants

S Nema1, K E Avis

  • 1Department of Pharmaceutics, University of Tennessee, Memphis.

Journal of Parenteral Science and Technology : a Publication of the Parenteral Drug Association
|March 1, 1993
PubMed

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...