[Immobilization of ferredoxin]

The method of pea ferredoxin (Fd) immobilization has been worked out. Under binding Fd with sepharose 4B activated by cyanogen bromide, a destruction took place in the Fd active center and apoprotein was bound with the matrix. The native immobilized Fd has been obtained when using P-300 biogel as a matrix. The gel was activated by glutaraldehyde and protein was linked with gel through a hexamethylenediamine spacer.