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Somatostatin receptor subtype gene expression in human and rodent tumors
1Biomeasure Inc, Milford, MA 01757-3650.
Abstract:
Somatostatin (SRIF) analogues display anti-tumor properties believed to be mediated by specific cell surface somatostatin receptors (SSTR). SSTR subtypes have unique pharmacological properties, including specific GTP-binding protein coupling, ion channel regulation, and cAMP inhibition; therefore, identification of isotypes expressed in tumor cells facilitates current efforts to design potent anti-tumor SRIF analogues. Human and rodent solid, transplantable tumors and tumor cell lines were examined for gene expression of SSTR1, SSTR2 and SSTR3 by reverse transcription of tumor mRNA and subsequent amplification of cDNA by the polymerase chain reaction, using SSTR subtype-specific oligonucleotide primers. SSTR2 mRNA transcripts were observed in all of the tumor cell lines examined. SSTR1 gene expression was seen in several human and rat tumor types, and SSTR3 gene expression observed in two rodent tumor types. SSTR mRNA-positive tumors are expected to possess membrane-bound receptors which could potentially interact with anti-tumor SRIF analogues.
Insights
Somatostatin receptor (SSTR) gene expression was analyzed in various tumor cells. SSTR2 was found in all tested cell lines, indicating potential targets for novel anti-tumor therapies.
Area of Science:
- Oncology
- Molecular Biology
- Pharmacology
Background:
- Somatostatin (SRIF) analogues exhibit anti-tumor effects.
- These effects are mediated by cell surface somatostatin receptors (SSTR).
- Different SSTR subtypes possess unique pharmacological properties.
Purpose of the Study:
- To identify somatostatin receptor (SSTR) subtypes expressed in tumor cells.
- To facilitate the design of potent anti-tumor SRIF analogues.
Main Methods:
- Examined gene expression of SSTR1, SSTR2, and SSTR3 in human and rodent tumors and cell lines.
- Utilized reverse transcription and polymerase chain reaction (PCR) with subtype-specific primers.
Main Results:
- SSTR2 mRNA transcripts were detected in all examined tumor cell lines.
- SSTR1 gene expression was observed in several human and rat tumor types.
- SSTR3 gene expression was found in two rodent tumor types.
Conclusions:
- Tumors expressing SSTR mRNA are likely to have membrane-bound receptors.
- These receptors can potentially interact with anti-tumor SRIF analogues.
- Findings support the development of targeted SSTR-based therapies.