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Application of DNA filter hybridization and PCR to distinguish between human and non-human tissues of poor quality
R Müllenbach1, D Makuch, H J Wagner
1Institut für Humangenetik, Universität des Saarlandes, Homburg/Saar, Germany.
International Journal of Legal Medicine
|January 1, 1993
Abstract:
The present report describes a novel approach for the identification of human or non-human specimens after long-term storage in a badly preserved state. The application of the PCR-technique (polymerase chain reaction) using human-specific primers as well as Southern blot (filter) hybridization of the sample DNA to a primate-specific DNA probe enabled us to extend the positive identification beyond the limits of conventional methods such as serological or morphological examinations.