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L-pipecolic acid oxidation in rat: subcellular localization and developmental study

V V Rao1, M J Tsai, X Pan

  • 1Department of Biochemistry, University of Maryland Dental School, Baltimore 21201-1586.

Biochimica Et Biophysica Acta
|June 24, 1993
PubMed
Summary
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L-pipecolic acid oxidase is found in rat liver mitochondria and peroxisomes. This enzyme

Area of Science:

  • Biochemistry
  • Cell Biology
  • Enzymology

Background:

  • L-pipecolic acid metabolism is crucial for biological processes.
  • Understanding the localization and regulation of L-pipecolic acid oxidase is essential.

Purpose of the Study:

  • To investigate the suborganellar localization of L-pipecolic acid oxidase in rat liver.
  • To characterize the enzyme's activity in different brain regions and its developmental profile.

Main Methods:

  • Sensitive radioactive assay method for enzyme activity measurement.
  • Suborganellar fractionation to determine enzyme localization.
  • Induction studies using glucagon and di-(2-ethylhexyl)phthalate.

Main Results:

Related Experiment Videos

  • L-pipecolic acid oxidase is localized in both mitochondria and peroxisomes of rat liver.
  • Brain white matter exhibits higher oxidation activity than brain cortex.
  • The enzyme is matrix-associated in mitochondria and membrane-associated in peroxisomes.
  • Activity is inducible by glucagon (liver) and di-(2-ethylhexyl)phthalate (brain).
  • Enzyme activity is detectable early in embryonic development, peaking at 15 days of gestation.

    • Conclusions:

    • L-pipecolic acid oxidase plays a significant role in both liver and brain tissues.
    • The enzyme's distinct localization and regulation suggest tissue-specific functions.
    • Its early developmental presence indicates importance in embryonic metabolism.