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Adenovirus isolations from patients with conjunctivitis in Thuringia

T Adrian1, H Brunnemann, R Wigand

  • 1Institut für Virologie und Seuchenhygiene, Medizinische Hochschule, Hannover, Germany.

Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|February 1, 1993
PubMed
Summary

This study identified 57 adenoviruses (AVs) from patients with conjunctivitis, with AV8 being the most common. DNA analysis revealed distinct genetic profiles for AV9 and AV17/H29 strains, aiding in their classification.

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Area of Science:

  • Ophthalmology
  • Virology
  • Molecular Biology

Background:

  • Adenoviruses (AVs) are a common cause of infectious conjunctivitis.
  • Understanding the genetic diversity of circulating AV strains is crucial for diagnosis and management.

Purpose of the Study:

  • To identify and genetically characterize adenoviruses isolated from patients with (kerato) conjunctivitis.
  • To analyze the genetic relatedness of different AV strains using DNA restriction analysis.

Main Methods:

  • Isolation and identification of 57 adenoviruses from patient samples collected between 1979 and 1986.
  • DNA restriction endonuclease analysis using five enzymes.
  • Physical mapping of restriction site alterations for specific enzymes and AV variants.

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Main Results:

  • Adenoviruses 8, 7, 3, 17/H29, 9, 2, and 10 were identified, with AV8 being the most prevalent.
  • Three intermediate AV17/H29 strains were found to be genetically identical.
  • Two AV9 strains showed variations from the prototype strain in two enzymes, with alterations mapped.
  • An AV8 variant with a HindIII alteration was also identified and mapped.

Conclusions:

  • The study provides a detailed genetic characterization of adenoviruses causing conjunctivitis during the specified period.
  • DNA restriction analysis is effective in differentiating and classifying AV strains.
  • Identified genetic variations in AV9 and AV8 strains highlight the importance of ongoing molecular surveillance.