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Dural neuropeptide changes after subarachnoid hemorrhage in rats
J T Keller1, B G Mullen, M Zuccarello
1University of Cincinnati College of Medicine, Department of Neurosurgery, OH 45267-0515.
Brain Research Bulletin
|January 1, 1993
Summary
Subarachnoid hemorrhage (SAH) alters neuropeptides in rat dura mater, reducing substance P and neuropeptide Y. Dural mast cells also show decreased serotonin (5-HT) after SAH.
Area of Science:
- Neuroscience
- Pathology
Background:
- Subarachnoid hemorrhage (SAH) is a critical neurological event.
- The impact of SAH on dural neuropeptides and mast cells remains largely uninvestigated.
Purpose of the Study:
- To investigate the effects of SAH on neuropeptides (CGRP, SP, NPY) and serotonin (5-HT) in the rat dura mater.
- To determine if dural nerves respond similarly to cerebral blood vessel nerves post-SAH.
Main Methods:
- SAH induced in rats via cisterna magna blood injection.
- Dural whole mounts analyzed using immunohistochemistry for CGRP, SP, NPY, and 5-HT at various time points (6h, 24h, 48h, 6 days).
Main Results:
- Substance P (SP) levels significantly decreased post-SAH, returning to baseline by day 6.
- Neuropeptide Y (NPY) innervation was markedly reduced, with slow recovery.
- Serotonin (5-HT) in dural mast cells decreased at 6 and 24 hours, normalizing by 48 hours.
- Calcitonin gene-related peptide (CGRP) immunostaining remained unchanged.
Conclusions:
- SAH differentially affects neuropeptide and mast cell content in the dura mater.
- The dura mater is a target for intracranial pathological processes like SAH.
- Differential neuronal responses or peptide release may explain observed changes.