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Related Experiment Videos

Denitrification by N2-fixing Sprillum lipoferum

C A Neyra, J Döbereiner

    Canadian Journal of Microbiology
    |March 1, 1977
    PubMed
    Summary

    This study found that nitrogen-fixing Spirillum lipoferum bacteria can reduce nitrate to nitrite and, in some cases, further reduce it to produce gas. This demonstrates a novel capability for denitrification in nitrogen-fixing bacteria.

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    International journal of systematic bacteriology·1996

    Area of Science:

    • Microbiology
    • Environmental Science
    • Biochemistry

    Background:

    • Nitrogen fixation is crucial for plant growth and soil fertility.
    • Denitrification is a key process in the nitrogen cycle, converting nitrates to nitrogen gas.
    • The ability of nitrogen-fixing bacteria to also perform denitrification is not well-understood.

    Purpose of the Study:

    • To investigate the nitrate-reducing and denitrifying capabilities of nitrogen-fixing Spirillum lipoferum strains.
    • To identify strains capable of reducing nitrate (NO3-) to nitrite (NO2-) and further to gaseous products.
    • To characterize the gaseous end-products of denitrification in these bacteria.

    Main Methods:

    • Isolation and identification of 49 N2-fixing Spirillum lipoferum strains from various plant roots and soils.
    • Assay of nitrate (NO3-) and nitrite (NO2-) reduction.
    • Gas production analysis, including the detection of nitrous oxide (N2O) and ethylene (C2H4) in the presence of acetylene (C2H2).

    Main Results:

    • All 49 strains reduced nitrate (NO3-) to nitrite (NO2-).
    • Thirty of these strains further reduced nitrite (NO2-) with significant gas production.
    • Representative denitrifying strains produced both nitrous oxide (N2O) and ethylene (C2H4), while non-denitrifying strains produced only ethylene (C2H4) in the presence of acetylene (C2H2).

    Conclusions:

    • This study reports the first instance of a nitrogen-fixing bacterium, Spirillum lipoferum, capable of denitrification.
    • The findings reveal diverse metabolic capabilities within N2-fixing bacteria, impacting nitrogen cycling.
    • These results have implications for understanding microbial contributions to soil nitrogen transformations.

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