Mg- and Ca-actin filaments appear virtually identical in steady-state as determined by dynamic light scattering

Dynamic light scattering measurements show that although Mg-actin polymerizes more rapidly than Ca-actin (actin at 0.04-0.4 mg/ml polymerized with 0.1 M KCl +/- 2 mM MgCl2 or CaCl2, at room temperature or at 10 degrees C), steady-state filaments exhibit nearly identical intensity autocorrelation functions and similar mean scattered intensities. The dynamic data are used to measure the persistence length of the filaments which is found to be 4.2 microns independent of the bound cation and of the actin concentration.