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Determination of argininosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography
S Canepari1, V Carunchio, A M Girelli
1Department of Chemistry, University of Rome La Sapienza, Italy.
Abstract:
A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samples.
