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Related Experiment Videos

Cytoplasmic coat proteins involved in endosome function

J A Whitney1, M Gomez, D Sheff

  • 1Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520-8002, USA.

Cell
|December 1, 1995
PubMed
Summary
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Endosomes recruit specific cytoplasmic coat proteins, including COP-I and ARF families, in a regulated manner. This protein recruitment is crucial for endosome function and transport during endocytosis.

Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Endosomes are central to receptor-mediated endocytosis, involving protein sorting and transport via vesicles.
  • Endosome function relies on the regulated assembly of cytoplasmic coat complexes.

Purpose of the Study:

  • To investigate the cytoplasmic protein recruitment to endosomes.
  • To identify the coat proteins involved in endosome function and transport.

Main Methods:

  • In vitro studies of endosome protein recruitment.
  • Stimulation with GTP gamma S and brefeldin A.
  • Analysis of recruited coat proteins, including COP-I, ARF, and clathrin-like complexes.

Main Results:

  • Endosomes recruit specific cytoplasmic proteins in a GTP gamma S-stimulated and brefeldin A-sensitive manner.

Related Experiment Videos

  • Recruited proteins include members of the COP-I and ARF families, as well as clathrin-like coats.
  • Antibodies to beta-COP inhibit viral entry via the endocytic pathway.

    • Conclusions:

    • Regulated assembly of cytoplasmic coat complexes governs endosome function.
    • COP-I or related proteins are essential for endosome function in intact cells.
    • Endosomes utilize distinct, clathrin-like coats for transport processes.