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The sequence of the rat pyruvate carboxylase-encoding cDNA
D A Lehn1, S M Moran, M J MacDonald
1Childrens Diabetes Center, University of Wisconsin, Madison 53706, USA.
Gene
|November 20, 1995
Summary
Researchers constructed a rat pyruvate carboxylase (PC) cDNA, revealing a protein sequence highly similar to mouse and human PC. This finding aids in understanding PC
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Pyruvate carboxylase (PC) is a crucial mitochondrial enzyme involved in gluconeogenesis and anaplerosis.
- Understanding the genetic and molecular basis of PC is vital for metabolic research.
Purpose of the Study:
- To construct and sequence a complementary DNA (cDNA) encoding rat pyruvate carboxylase (PC).
- To compare the deduced amino acid sequence of rat PC with its counterparts in other species.
Main Methods:
- Isolation of cDNA clones from a rat liver cell cDNA library.
- Construction of a composite 3945-bp cDNA sequence.
- Determination of the nucleotide sequence.
- Bioinformatic analysis of the open reading frame and amino acid sequence.
Main Results:
- A complete 3945-bp cDNA sequence for rat pyruvate carboxylase (PC) was successfully constructed.
- The open reading frame encodes a protein of 1178 amino acids.
- The rat PC protein sequence exhibits high identity (98.6%) and similarity (99.0%) to mouse PC.
- Rat PC also shows significant identity (96.0%) and similarity (97.8%) to human PC.
Conclusions:
- The successful construction and sequencing of rat PC cDNA provide a valuable resource for further research.
- The high conservation of the PC protein across species suggests a critical and conserved biological function.
- This work facilitates comparative studies of pyruvate carboxylase function and evolution.