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Related Experiment Videos

Rab 7: an important regulator of late endocytic membrane traffic

Y Feng1, B Press, A Wandinger-Ness

  • 1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500, USA.

The Journal of Cell Biology
|December 1, 1995
PubMed
Summary

Rab7 protein is crucial for transporting molecules from early to late endosomes, a process essential for cellular degradation. This study clarifies rab7

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Endocytosis and intracellular trafficking

Background:

  • Rab5 protein regulates early endocytosis.
  • The function of rab7 protein in the endocytic pathway was previously uncharacterized.
  • Rab proteins are small GTPases involved in vesicular transport.

Purpose of the Study:

  • To investigate the function of rab7 protein in the endocytic pathway.
  • To determine the role of rab7 in the transport of proteins from early to late endosomes.

Main Methods:

  • Overexpression of wild-type and dominant-negative mutant rab7 proteins (rab7N125I, rab7T22N) in baby hamster kidney cells.
  • Analysis of vesicular stomatitis virus (VSV) G protein and paramyxovirus SV5 hemagglutinin-neuraminidase (HN) protein trafficking.

Related Experiment Videos

  • Quantitative biochemical assays using horseradish peroxidase.

    • Main Results:

    • Overexpression of wild-type rab7 facilitated normal transport to late endosomes.
    • Mutant rab7 proteins caused accumulation of VSV G protein in early endosomes.
    • Mutant rab7 proteins inhibited the cleavage of SV5 HN protein, indicating a role in downstream degradative events.

    Conclusions:

    • Rab7 plays a key role in regulating membrane transport from early to late endosomes, downstream of rab5.
    • Rab7 function is essential for degradative events in the late endosome.
    • Findings are comparable to yeast homologues, suggesting conserved function.