[Effect of sex steroids on serum amyloid P-component (female protein) in rats]

Serum amyloid P-component (SAP) has been designated as a female protein in hamsters. But such a distinction is not made for rats. In order to investigate the effects of sex-steroids on the SAP level in rats, SAP was purified from Wistar rats by affinity chromatography of phosphorylcholine, followed by gel filtration. Anti-SAP was raised through the immunization of rabbits with the rat SAP and Freund's adjuvant. Sample sera were obtained from 180 young and old rats, after which rats were injected with estradiol (E2), testosterone (T) or dehydroepiandrosterone (DHEA). Sera were serially obtained from the tail vessels until the 8th day after injection. The SAP level was assayed by micro single radial immunodiffusion. As the rats aged, the SAP levels increased from 2.9 mg/dl at 11 weeks to 10.7mg/dl at 58 weeks. In 37-week-old rats, the SAP levels in females (6.3 +/- 1.8 mg/dl) were significantly (p < 0.001) higher than those in males (3.9 +/- 1.0 mg/dl). The SAP levels did not change after T administration, but were increased rapidly by E2 administration, especially in young male rats (increased from 2.6 +/- 0.2 mg/dl to 4.9 +/- 0.7 mg/dl). The SAP levels were decreased significantly (p < 0.05) by DHEA injection. Serum E2 levels in young (11 wk) male rats were very low before E2 injection, and rose steeply on the 2nd day. From these findings, the different SAP levels in mature female and male rats are attributed to E2.