Related Experiment Videos
Studies on the replication of Mayaro virus grown in interferon treated cells
M C Rebello1, M E Fonseca, J O Marinho
1Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciéncias de Saúde, Brasil.
Abstract:
Mayaro virus grown in interferon treated infected cells has been characterized with regard to its ability to replicate in vertebrate (TC7) and invertebrate (Aedes albopictus) cells. Virus purified from interferon treated TC7 cells adsorbs and penetrates to the same extent as the control virus. During infection, these virus particles caused inhibition of host protein synthesis and synthesized the same spectrum of viral proteins as normal virus. This population however, was apparently more sensitive to interferon treatment. Electron microscopy of TC7 cells showed the presence of numerous aberrant virus particles budding from the plasma membrane.
Insights
Mayaro virus grown in interferon-treated cells showed similar replication but increased sensitivity to interferon. Aberrant virus particles were observed budding from infected cells, indicating potential replication defects.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Mayaro virus (MAYV) is an arbovirus with significant public health implications.
- Interferon (IFN) is a key component of the innate immune system that restricts viral replication.
- Understanding virus-host interactions, especially under immune pressure, is crucial for antiviral strategies.
Purpose of the Study:
- To characterize Mayaro virus grown in interferon-treated vertebrate (TC7) and invertebrate (Aedes albopictus) cells.
- To investigate the replication, protein synthesis, and physical characteristics of MAYV produced under IFN influence.
- To assess the impact of interferon treatment on MAYV infectivity and particle morphology.
Main Methods:
- Virus propagation in TC7 and Aedes albopictus cells with and without prior interferon treatment.
- Adsorption and penetration assays to evaluate viral entry efficiency.
- Analysis of host protein synthesis inhibition and viral protein production via SDS-PAGE.
- Electron microscopy to visualize virus particle morphology and budding process.
Main Results:
- Virus grown in interferon-treated cells exhibited similar adsorption and penetration capabilities compared to control virus.
- These MAYV particles effectively inhibited host protein synthesis and produced a comparable spectrum of viral proteins.
- However, the virus population derived from interferon-treated cells demonstrated increased sensitivity to interferon.
- Electron microscopy revealed numerous aberrant virus particles budding from the plasma membrane of TC7 cells.
Conclusions:
- Interferon treatment of infected cells generates MAYV with altered properties, including enhanced interferon sensitivity.
- The observed aberrant viral particles suggest potential defects in viral assembly or maturation under interferon pressure.
- These findings provide insights into Mayaro virus adaptation and replication dynamics in the context of innate immune responses.