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Multilocus enzyme electrophoresis study of Bacillus sphaericus
1Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
Memorias Do Instituto Oswaldo Cruz
|January 1, 1995
Summary
Multilocus enzyme electrophoresis (MLEE) identified a unique allele (NP-4) distinguishing Bacillus sphaericus. Entomopathogenic strains form a clonal population with two dominant genotypes, indicating genetic uniformity.
Area of Science:
- Microbiology
- Genetics
- Biochemistry
Background:
- Multilocus enzyme electrophoresis (MLEE) is a valuable tool for microbial population genetics.
- The Bacillus sphaericus group includes important entomopathogenic species, but their genetic diversity requires further investigation.
Purpose of the Study:
- To apply MLEE and numerical analysis to study the genetic diversity within the Bacillus sphaericus group.
- To identify genetic markers for distinguishing entomopathogenic B. sphaericus strains.
Main Methods:
- Multilocus enzyme electrophoresis (MLEE) was performed on various Bacillus strains.
- Numerical analysis was used to cluster strains based on their electrophoretic profiles.
- Specific alleles (NP-4 and ADH-2) and genotypes (zymovar 59 and zymovar 119) were identified.
Main Results:
- A unique allele (NP-4) was identified, distinguishing B. sphaericus from other entomopathogenic Bacillus species.
- All entomopathogenic B. sphaericus strains clustered together, sharing a common allele (ADH-2).
- The entomopathogenic B. sphaericus population appears clonal, characterized by two prevalent genotypes: zymovar 59 and zymovar 119.
Conclusions:
- MLEE effectively differentiates B. sphaericus and its entomopathogenic relatives.
- Entomopathogenic B. sphaericus strains exhibit limited genetic diversity, suggesting a clonal population structure.
- The identified genetic markers can aid in the identification and characterization of entomopathogenic B. sphaericus.