Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prophage distribution in coryneform bacteria

S Moreau1, V Leret, C Le Marrec

  • 1Laboratoire de Génétique et Physiologie microbiennes, Rennes, France.

Research in Microbiology
|July 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Claudin 18.2 expression in gastroesophageal adenocarcinoma: biomarker overlap and association with clinical outcomes in a European cohort.

ESMO open·2026
Same author

Assessment of an Allergy Study Including Rapid Provocation Tests to Identify a Safe Alternative Contrast Agent and Phenotyping of Patients With a History of Hypersensitivity to Iodinated Contrast Media.

Journal of investigational allergology & clinical immunology·2025
Same author

Coupons and state tobacco policy context as predictors of tobacco use among those reporting e-cigarette use in the U.S.: Findings from the Population Assessment of Tobacco and Health (PATH) Study waves 5-6 (2018-2021).

Preventive medicine reports·2025
Same author

ATAK Complex Induced by Ranitidine: A Comprehensive Concept for Cardiac Involvement in Allergic Reactions.

Journal of investigational allergology & clinical immunology·2024
Same author

DNA demethylation and hypermethylation are both required for late nodule development in Medicago.

Nature plants·2022
Same author

A Reply to "Spanish Society of Allergology and Clinical Immunology (SEAIC) Vision of Drug Provocation Tests".

Journal of investigational allergology & clinical immunology·2022
Same journal

Deletion of major shell proteins of ethanolamine utilization microcompartment reduces intrinsic antibiotic resistance, biofilm, and intracellular survival of Salmonella Typhimurium.

Research in microbiology·2025
Same journal

Dynamic exometabolomics reveals metabolic adaptations of Staphylococcus epidermidis to pH-mimicking skin and bloodstream.

Research in microbiology·2025
Same journal

Co-expression network insights into Leptospira interrogans pathogenesis.

Research in microbiology·2025
Same journal

Differential DNA methylation associated with virulence attenuation in Edwardsiella piscicida.

Research in microbiology·2025
Same journal

Klebsiella pneumoniae-induced pneumonia: Pathogenesis, immune interactions, and antimicrobial resistance in a global context.

Research in microbiology·2025
Same journal

Investigating inhibitory effect of sterol targeting compounds against B. anthracis: Membrane microdomain a probable target.

Research in microbiology·2025
See all related articles

Four temperate bacteriophages were isolated from Corynebacterium strains. These Siphoviridae family phages have double-stranded DNA genomes and integrate into host chromosomes, with one persisting freely.

Area of Science:

  • Microbiology
  • Virology
  • Bacteriophage Research

Background:

  • Temperate bacteriophages are viruses that can integrate their genome into the host bacterium's chromosome.
  • Corynebacterium species are important industrial microorganisms, and understanding their associated phages is crucial for strain stability and genetic manipulation.
  • UV induction is a common method to activate prophages within bacterial hosts.

Purpose of the Study:

  • To isolate and characterize novel temperate bacteriophages from Corynebacterium species.
  • To investigate the host range and genomic properties of these isolated phages.
  • To determine the integration mechanism and genomic homology of the induced prophages.

Main Methods:

  • Isolation of bacteriophages from Corynebacterium strains using UV induction.

Related Experiment Videos

  • Plaque assays to determine phage host range.
  • Genome analysis including DNA type (double-stranded DNA) and cohesive ends.
  • Prophage integration site analysis (chromosomal vs. free).
  • DNA-DNA hybridization for homology studies.

    • Main Results:

    • Four temperate bacteriophages (phi 304L, phi 304S, phi 15, phi 16) were isolated from various Corynebacterium glutamicum and Corynebacterium sp. strains.
    • Phages phi 304L and phi 304S had no known sensitive hosts, while phi 15 and phi 16 exhibited specific plaque formation on certain Corynebacterium strains.
    • All isolated phages belong to the Siphoviridae family and possess double-stranded DNA genomes with cohesive ends, showing no inter-phage homology.
    • Prophages phi 16, phi 304L, and phi 304S integrated into host chromosomes, whereas prophage phi 15 appeared to exist episomally.
    • Cross-hybridization confirmed the absence of these prophages in other tested Corynebacterium and Brevibacterium strains.

    Conclusions:

    • Characterization of four novel temperate bacteriophages from Corynebacterium species.
    • Demonstration of diverse integration strategies (chromosomal vs. episomal) among these phages.
    • Confirmation of the genetic distinctness of these phages and their specific association with their host strains.