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Related Experiment Videos

Rapid microplate mitogenic assay using a meltable scintillation wax

R Hinrichs1, M Ueffing

  • 1Institut für Klinische Molekularbiologie und Tumorgenetik GSF, München, Germany.

Biotechniques
|August 1, 1995
PubMed
Summary

A novel in-plate cell proliferation assay using scintillation wax offers a safer, more convenient alternative to traditional liquid scintillation. This method provides comparable results for [3H]thymidine incorporation, simplifying cell-based assays.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Biochemistry

Background:

  • Traditional liquid scintillation assays generate hazardous waste.
  • 96-well plate formats are convenient for cell-based studies.
  • Monitoring cell morphology alongside proliferation is advantageous.

Purpose of the Study:

  • To develop a safer and more convenient in-plate cell proliferation assay.
  • To combine solid scintillation advantages with 96-well plate technology.
  • To validate a new assay using scintillation wax for [3H]thymidine incorporation.

Main Methods:

  • An in-plate cell proliferation assay was designed using scintillation wax (FlexiScint).
  • The assay utilized 96-well ViewPlates for simultaneous cell counting and morphology monitoring.

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  • [3H]thymidine incorporation was measured in an adherent cell line and compared to liquid scintillation.

    • Main Results:

    • The scintillation wax assay produced results comparable to conventional liquid scintillation counting.
    • The new method eliminates messy liquid waste, enhancing safety and convenience.
    • In-plate counting allowed for monitoring of cell morphology during experiments.

    Conclusions:

    • The developed in-plate scintillation wax assay is a viable and advantageous alternative to liquid scintillation.
    • This method simplifies cell proliferation studies, improving safety and convenience.
    • The assay enables simultaneous assessment of cell proliferation and morphology.