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Related Experiment Videos

Combining optical and atomic force microscopy for life sciences research

J Vesenka1, C Mosher, S Schaus

  • 1Iowa State University, Ames, USA.

Biotechniques
|August 1, 1995
PubMed
Summary
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The BioScope, a combined atomic force microscope and confocal optical microscope, achieves high-resolution imaging of biological samples under physiological conditions. This powerful tool visualizes both internal structures and surface morphology with nanoscale precision.

Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Atomic Force Microscopy (AFM) provides nanoscale surface topography.
  • Confocal optical microscopy offers sub-cellular imaging.
  • Simultaneous high-resolution imaging of biological samples is challenging.

Purpose of the Study:

  • To integrate AFM with confocal optical microscopy.
  • To develop a robust system for imaging biological specimens under physiological conditions.
  • To demonstrate the capabilities of the combined system for nanoscale and sub-cellular imaging.

Main Methods:

  • Combined atomic force microscopy (AFM) and confocal optical microscopy into a single instrument (BioScope).
  • Utilized fluorescent markers for internal structure visualization.

Related Experiment Videos

  • Performed imaging of biological samples, including living cells and DNA, under physiological conditions.

    • Main Results:

    • Achieved 20 nm resolution for surface morphology and 200 nm resolution for internal structure.
    • Successfully imaged living Xenopus retinal glial (XR1) cells, Drosophila polytene chromosomes, and colloidal gold-labeled plasmid DNA.
    • Demonstrated simultaneous imaging of surface and internal features of biological specimens.

    Conclusions:

    • The BioScope system enables high-resolution, correlative imaging of biological samples.
    • This integrated approach allows for detailed analysis of cellular and molecular structures under near-native conditions.
    • The BioScope is a versatile tool for advancing research in cell biology and biophysics.