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Related Experiment Videos

Hydrostatic pressure-induced conformational changes in phosphatidylcholine headgroups: a 2H NMR study

B B Bonev1, M R Morrow

  • 1Department of Physics, Memorial University of Newfoundland, St. John's, Canada.

Biophysical Journal
|August 1, 1995
PubMed
Summary

Pressure alters phospholipid headgroup conformations, affecting choline deuteron quadrupole splittings. These changes, observed in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dimyristoyl-sn-glycero-3-phosphocholine, indicate headgroup tilting and reduced area per lipid.

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Area of Science:

  • Biophysics
  • Materials Science
  • Physical Chemistry

Background:

  • Phospholipids like DPPC and DMPC form lipid bilayers crucial for cell membranes.
  • Headgroup conformation influences membrane properties and interactions.
  • Deuterium NMR spectroscopy is a powerful tool for probing molecular dynamics and structure.

Purpose of the Study:

  • To investigate the impact of pressure and temperature on the headgroup conformations of DPPC and DMPC.
  • To correlate observed changes with lipid packing and surface charge effects.
  • To elucidate the molecular mechanisms underlying pressure-induced alterations in phospholipid behavior.

Main Methods:

  • Deuterium nuclear magnetic resonance (NMR) spectroscopy was employed.
  • Isothermal compression experiments were conducted.

Related Experiment Videos

  • Temperature-dependent NMR measurements were performed at ambient and elevated pressures.

    • Main Results:

    • Isothermal compression decreased alpha deuteron quadrupole splitting and increased beta and gamma splittings.
    • These changes mirror those seen with positive surface charge, suggesting headgroup tilting.
    • Elevated pressure made beta and gamma splittings less sensitive to temperature variations.

    Conclusions:

    • Pressure induces headgroup tilting in DPPC and DMPC, consistent with reduced area per lipid.
    • Temperature effects on headgroup conformation are modulated by pressure.
    • NMR provides insights into the complex interplay of pressure, temperature, and lipid headgroup structure.