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Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Vibrio mimicus
F N Musayev1, Y L Lee, J F Shaw
1Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
Protein Science : a Publication of the Protein Society
|September 1, 1995
Abstract:
Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53.46(1) A, c = 65.79(1) A, and beta = 106.54(1) degrees. There are two molecules of molecular weight 22 kDa in an asymmetric unit with a solvent content of 43%.