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Viable porcine hepatocytes from slaughterhouse organs
H G Koebe1, J Mueller-Hoecker, S Koebe
1Department of Surgery, Klinikum Grosshadern, L.M. University of Munich, F.R. Germany.
Abstract:
A modified enzymatic isolation technique for the successful harvesting of porcine liver cells from slaughterhouse organs is introduced. Digestion of the left medial liver lobe (n = 74) resulted in 1.0 +/- 0.3 x 10E7 viable hepatocytes per gram tissue and an overall yield of 1.92 +/- 0.5 x 10E9 cells per isolation (viability: 93 +/- 2%).