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Phage display: protein engineering by directed evolution

K T O'Neil¹, R H Hoess

¹DuPont Merck Pharmaceutical Company, Chemical and Physical Sciences Department, Wilmington, DE 19880-0328, USA.

Current Opinion in Structural Biology

|August 1, 1995

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Phage display technology is advancing protein engineering. Recent applications include developing DNA-binding proteins, studying protein folding, evolving antibodies, and analyzing protein-protein interactions using cDNA libraries.

Area of Science:

Biochemistry
Molecular Biology
Protein Engineering

Background:

Phage display is a powerful technique for protein engineering.
Its applications have significantly expanded in recent years.

Purpose of the Study:

To highlight the expanded versatility of phage display technology.
To showcase novel applications in protein engineering and interaction studies.

Main Methods:

Phage display of proteins.
Development of DNA-binding proteins.
Analysis of protein folding energetics.
Directed evolution of antibodies.
Display of expressed cDNA libraries for protein-protein interaction studies.

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Main Results:

Phage display enables the development of DNA-binding proteins with new specificities.
The technology aids in understanding protein folding energetics.
Directed evolution of antibodies is effectively achieved using phage display.
Expressed cDNA libraries on phage facilitate the study of protein-protein interactions.

Conclusions:

Phage display technology continues to evolve, offering versatile tools for protein engineering.
Its expanded applications provide new avenues for research in molecular biology and drug discovery.