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Multiple cloning sites carrying loxP and FRT recognition sites for the Cre and Flp site-specific recombinases
M R Snaith1, J A Murray, C A Boulter
1Department of Genetics, University of Cambridge, UK.
Gene
|December 1, 1995
Abstract:
Plasmids were constructed carrying the loxP and FRT recognition sites for the Cre and Flp site-specific recombinases, respectively, within multiple cloning sites. Vectors carrying single and tandemly repeated targets are available with various flanking restriction enzyme sites. In addition, a series of plasmids carrying both loxP and FRT sites is available. These vectors facilitate construction of target molecules for these site-specific recombinases which are becoming increasingly important tools for the in vivo manipulation of DNA.