Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Soluble multimeric Alzheimer beta(1-40) pre-amyloid complexes in dilute solution

H Levine1

  • 1Department of Neurodegenerative Diseases, Parke-Davis Pharmaceutical Research Division of the Warner-Lambert Company, Ann Arbor, MI 48106-1047, USA.

Neurobiology of Aging
|September 1, 1995
PubMed
Summary

Beta(1-40) peptide forms soluble pentameric/hexameric complexes that aggregate into amyloid structures under specific conditions. This cooperative growth mechanism may explain plaque accretion and neurotoxicity variations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantum optimization of maximum independent set using Rydberg atom arrays.

Science (New York, N.Y.)·2022
Same author

Probing topological spin liquids on a programmable quantum simulator.

Science (New York, N.Y.)·2021
Same author

Controlling quantum many-body dynamics in driven Rydberg atom arrays.

Science (New York, N.Y.)·2021
Same author

Historic tobacco legislation in Israel: a moment to celebrate.

Israel journal of health policy research·2020
Same author

Generation and manipulation of Schrödinger cat states in Rydberg atom arrays.

Science (New York, N.Y.)·2019
Same author

The Q359K/T360K mutation causes cystic fibrosis in Georgian Jews.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2018

Area of Science:

  • Biochemistry
  • Neuroscience
  • Materials Science

Background:

  • Amyloidogenic peptides like beta(1-40) are implicated in neurodegenerative diseases.
  • Understanding the early aggregation states of peptides is crucial for disease mechanism elucidation.

Purpose of the Study:

  • To investigate the spontaneous aggregation of beta(1-40) peptide in aqueous solutions.
  • To characterize the formation and properties of preamyloid structures under varying conditions.

Main Methods:

  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after glutaraldehyde and borohydride treatment.
  • Thioflavine T fluorescence assays to detect beta-sheet structures.
  • Sedimentation and filtration studies to assess aggregate properties.

Related Experiment Videos

Main Results:

  • Beta(1-40) peptide spontaneously forms pentameric/hexameric complexes in aqueous solution.
  • Under amyloidogenic conditions (low pH, high concentration), these complexes form sedimentable and filterable structures with beta-sheet characteristics.
  • Preamyloid structures are present even at low peptide concentrations, indicating cooperative growth.

Conclusions:

  • A monomer-multimer equilibrium exists for beta(1-40) peptide, influencing its aggregation pathway.
  • Cooperative growth of preamyloid structures, rather than solely monomeric addition, may drive amyloid plaque formation.
  • These findings offer insights into the variable biological consequences, including neurotoxicity, associated with amyloidogenic processes.