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Updated: Aug 15, 2026

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
Published on: March 27, 2021
Three dimensional observation of whole mount cultured cells stained with histochemical reactions by ultrahigh voltage
1Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
Abstract:
Thick biological specimens prepared as whole mount cultured cells stained with histochemical reactions, such as thiamine pyrophosphatase, glucose-6-phosphatase, cytochrome oxidase, acid phosphatase, DAB reactions demonstrating specific cell organelles such as Golgi apparatus, endoplasmic reticulum, mitochondria, lysosomes, peroxisomes and pinocytotic vesicles, were observed by ultrahigh voltage electron microscopy at accelerating voltages of 400-1000 kV producing stereo-pairs. As a result, those cell organelles were observed 3-dimensionally and the relative relationships between these organelles demonstrated.
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