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GABA uptake in a Saccharomyces cerevisiae strain
M Bermúdez Moretti1, S Correa García, E H Ramos
1Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONICET, FCEyN, UBA), Ciudad Universitaria, Buenos Aires, Argentina.
Cellular and Molecular Biology (Noisy-Le-Grand, France)
|September 1, 1995
Summary
This study investigated 4-aminobutyric acid (GABA) transport in Saccharomyces cerevisiae, finding that 5-aminolevulinic acid (ALA) competitively inhibits GABA uptake, which is regulated by carbon source. GABA entry was minimal when cells were pre-grown with GABA.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- 4-aminobutyric acid (GABA) is a crucial neurotransmitter and metabolic intermediate.
- Understanding GABA transport in yeast is essential for metabolic engineering and biotechnological applications.
- The specific permeases involved in GABA uptake in Saccharomyces cerevisiae strains like D27 require detailed characterization.
Purpose of the Study:
- To characterize the transport of 4-aminobutyric acid (GABA) in the Saccharomyces cerevisiae D27 strain.
- To investigate the relationship between 5-aminolevulinic acid (ALA) and GABA transport systems.
- To elucidate the regulatory mechanisms governing GABA incorporation in yeast.
Main Methods:
- Utilized kinetic studies to analyze GABA uptake.
- Investigated the effect of 5-aminolevulinic acid (ALA) on GABA transport.
- Examined the influence of carbon and nitrogen sources on GABA incorporation.
- Assessed GABA uptake in yeast grown in the presence of GABA.
Main Results:
- The general amino acid permease (GAP) is inactive in the D27 strain.
- GABA uptake is primarily mediated by a single transport system, not exclusively by PUT4 and UGA4 permeases as initially hypothesized.
- 5-aminolevulinic acid (ALA) competitively inhibits GABA uptake.
- GABA incorporation is regulated by the carbon source but not the nitrogen source.
- Yeast cells exhibited very low GABA entrance when pre-grown in the presence of GABA.
Conclusions:
- The characterization of GABA transport in Saccharomyces cerevisiae D27 reveals a complex system potentially involving novel permeases.
- The competitive inhibition of GABA uptake by ALA suggests a shared transport mechanism or regulatory interaction.
- Carbon source availability significantly influences GABA incorporation, highlighting its metabolic regulation.
- Low GABA uptake upon pre-growth indicates adaptive mechanisms or feedback inhibition in yeast.