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Related Experiment Videos

The clonogenic precursor cell in multiple myeloma

M H Bakkus1, I Van Riet, C De Greef

  • 1Dept. of Hematology-Immunology, Medical School, Vrije Universiteit Brussel (VUB), Belgium.

Leukemia & Lymphoma
|July 1, 1995
PubMed
Summary

Multiple myeloma research suggests the oncogenic event originates in mature B cells, not immature ones. Analyzing immunoglobulin genes helps identify these precursor cells, like memory B cells or plasmablasts.

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Detection of Interleukin-1β and Interleukin-6 in Human Multiple Myeloma by Fluorescent in situ Hybridization.

Leukemia & lymphoma·2016

Area of Science:

  • Hematology
  • Oncology
  • Immunology

Background:

  • Multiple myeloma is a cancer of plasma cells in the bone marrow.
  • The exact precursor cell for multiple myeloma remains controversial.
  • Diverse cell phenotypes are observed in patients.

Purpose of the Study:

  • To investigate the B-cell differentiation stage where multiple myeloma originates.
  • To utilize immunoglobulin gene sequencing for identifying precursor cells.

Main Methods:

  • Phenotypic analysis of bone marrow and peripheral blood.
  • Recombinant DNA technology to create clonal markers.
  • Sequencing of myeloma immunoglobulin genes.

Main Results:

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  • Lack of a myeloma-specific genetic lesion complicates precursor identification.
  • Immunoglobulin gene sequencing provides insights into B-cell differentiation stage.
  • Somatic mutations suggest the precursor is a mature B cell (memory B cell or plasmablast), not a pre-B cell or stem cell.

    • Conclusions:

    • The oncogenic transformation in multiple myeloma likely occurs in mature B cells.
    • Memory B cells or plasmablasts are probable precursor cells.
    • Immunoglobulin gene analysis is crucial for understanding myeloma origins.