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Related Experiment Videos

Novel mouse microsatellites: primer sequences and chromosomal location

T Suda1, M Oyanagi, S Wakana

  • 1First Department of Biochemistry, Niigata University School of Medicine, Japan.

DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
|January 1, 1994
PubMed
Summary

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Researchers identified 69 mouse DNA sequences with microsatellites, finding 51 with size variations useful for genetic mapping. These novel microsatellite markers are now located on most mouse chromosomes, aiding future genome studies.

Area of Science:

  • Genomics
  • Molecular Biology
  • Genetics

Background:

  • Microsatellites are repetitive DNA sequences.
  • Microsatellite markers are valuable tools in genetic mapping.
  • Developing new markers is crucial for advancing genome research.

Purpose of the Study:

  • To identify and characterize novel microsatellite markers from mouse genomic DNA.
  • To determine the chromosomal locations of these microsatellite markers.
  • To assess the utility of these markers for genetic and genome mapping.

Main Methods:

  • Genomic DNA from mouse strains (C57BL/6 and MSM) was cloned into a pUC118 library.
  • Microsatellite-containing sequences were identified and analyzed.
  • Polymerase chain reaction (PCR) and gel electrophoresis were used to detect size variations.

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  • Chromosomal mapping was performed using a backcross mouse panel typed with 85 anchor loci.

    • Main Results:

    • Sixty-nine microsatellite-containing sequences were identified.
    • Fifty-one sequences exhibited allelic size variations between the two mouse strains.
    • These polymorphic microsatellites were mapped to most mouse chromosomes, excluding chromosomes 16 and 19.
    • Novel microsatellite markers with defined chromosomal locations were generated.

    Conclusions:

    • The identified microsatellites represent valuable new genetic markers for the mouse genome.
    • These markers facilitate linkage analysis and enhance the resolution of genome mapping studies.
    • The defined locations of these markers contribute to a more comprehensive understanding of mouse chromosome organization.