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Organization of paramutagenicity in R-stippled maize

J L Kermicle1, W B Eggleston, M Alleman

  • 1Laboratory of Genetics, University of Wisconsin, Madison 53706, USA.

Genetics
|September 1, 1995
PubMed
Summary
This summary is machine-generated.

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The R-stippled (R-st) genetic element heritably reduces pigmenting potential through paramutation. Its paramutagenic strength depends on the number of r genes, with single genes being inactive.

Area of Science:

  • Genetics
  • Epigenetics
  • Molecular Biology

Background:

  • Paramutation is a heritable gene regulation phenomenon.
  • The R-stippled (R-st) allele in maize influences pigmenting potential.
  • R-st is composed of four tandemly arranged r genes.

Purpose of the Study:

  • To investigate the relationship between R-st gene copy number and paramutagenic strength.
  • To determine the role of individual r genes and intragenic regions in paramutation.
  • To elucidate the functional unit of R-st in mediating allelic modification.

Main Methods:

  • Analysis of deletion products generated by unequal crossing over within R-st.
  • Assessing paramutagenic activity of R-st derivatives with varying gene copy numbers.

Related Experiment Videos

  • Investigating the effect of intragenic recombination on paramutagenicity.

    • Main Results:

    • Paramutagenic strength decreased proportionally with the reduction in r gene copy number.
    • Single-gene R-st derivatives, including those with the I-R element, were nonparamutagenic.
    • Recombination restoring multiple r genes increased paramutagenicity, correlating with gene number.
    • Overlapping deletions retained moderate activity, indicating no single r gene is essential.
    • Two-gene derivatives were less paramutagenic in trans than in cis, suggesting cis-dependency.

    Conclusions:

    • The paramutagenic strength of R-st is directly related to the number of r genes present.
    • Multiple r genes within R-st function interdependently in a cis configuration for effective paramutation.
    • No single r gene or specific intragenic region is solely responsible for paramutagenicity.