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Published on: November 15, 2017
Detection of maize DNA sequences amplified in wheat
1Department of Plant Pathology, Kansas State University, Manhattan 66506-5502, USA.
Genome
|October 1, 1995
Summary
Genomic in situ hybridization detected amplified maize DNA sequences in hexaploid wheat chromosomes. These findings highlight the integration and location of foreign DNA within the wheat genome.
Area of Science:
- Genetics
- Molecular Biology
- Plant Science
Background:
- Genomic instability and DNA sequence integration are crucial in plant evolution.
- Understanding interspecific DNA transfer is key to crop improvement.
Purpose of the Study:
- To investigate the presence and location of amplified maize DNA sequences in hexaploid wheat cv. Chinese Spring.
- To analyze the distribution of these sequences across different wheat chromosomes.
Main Methods:
- Genomic in situ hybridization (GISH) was performed on somatic metaphase chromosomes of hexaploid wheat.
- Biotinylated maize genomic DNA was used as a probe for hybridization.
Main Results:
- Amplified maize DNA sequences were identified on five pairs of chromosomes in hexaploid wheat (1A, 7A, 2B, 3B, and 7B).
- Hybridization sites were also observed in hexaploid oat.
- Significant variation in the location and size of in situ hybridization sites was noted among progenitor species.
Conclusions:
- The study confirms the presence of integrated maize DNA in the wheat genome.
- The findings provide insights into chromosome organization and potential horizontal gene transfer in cereals.
- Variations in hybridization sites suggest differential integration or rearrangement events in related species.
