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Related Experiment Videos

Mapping of digested and undigested random amplified microsatellite polymorphisms in barley

J Becker1, M Heun

  • 1Max-Planck-Institut für Züchtungsforschung, Köln, Germany.

Genome
|October 1, 1995
PubMed
Summary

Random amplified microsatellite polymorphisms (RAMPs) offer a new method for barley genetic mapping. These DNA markers effectively expanded the barley linkage map, providing valuable tools for plant genetics research.

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Microsatellite markers are crucial for plant genetic mapping but require sequence data.
  • This limits their broad application in species like barley.

Purpose of the Study:

  • To explore random amplified microsatellite polymorphisms (RAMPs) as an alternative for barley genetic mapping.
  • To develop and map new DNA markers for the barley genome.

Main Methods:

  • RAMPs were generated by combining (GA)n microsatellite primers with random amplified polymorphic DNA (RAPD) primers.
  • Digestion with MseI created additional polymorphisms (dRAMPs).
  • Sixty RAMPs/dRAMPs identifying 40 new loci were mapped onto a barley RFLP map.

Main Results:

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  • RAMPs and dRAMPs identified 40 new genetic loci in barley.
  • These markers are distributed across all barley chromosomes.
  • The barley linkage map was extended by 174 cM to a total length of 1270 cM.

Conclusions:

  • RAMPs and dRAMPs are effective tools for barley genetic mapping.
  • These markers significantly enhance the resolution and coverage of the barley genetic map.
  • Observed genome stretching effects with RAMPs/dRAMPs mirror those seen with AFLPs, suggesting conserved mechanisms.