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Changes of MAP2 phosphorylation during brain development

B M Riederer1, E Draberova, V Viklicky

  • 1Institute of Anatomy, University of Lausanne, Switzerland.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|December 1, 1995
PubMed
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Monoclonal antibodies (MAbs) targeting microtubule-associated protein 2 (MAP2) show varied detection of its isoforms. Antibody differences highlight phosphorylation and conformational changes in MAP2, crucial for neuronal development and function.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Immunocytochemistry

Background:

  • Microtubule-associated protein 2 (MAP2) is vital for neuronal morphology during development and in adulthood.
  • Multiple MAP2 splice variants (MAP2a-d) exist, with MAP2a absent in feline brains.
  • MAP2 serves as a widely recognized neuronal marker.

Purpose of the Study:

  • To compare the immunocytochemical distribution of MAP2 isoforms using five distinct monoclonal antibodies (MAbs).
  • To investigate how phosphorylation and conformational changes affect MAP2 detection by different MAbs.
  • To analyze temporal and regional differences in MAP2 expression during feline visual cortex and cerebellum development.

Main Methods:

  • Comparison of five monoclonal antibodies (MAbs) against MAP2.

Related Experiment Videos

  • Immunocytochemical analysis of MAP2 distribution in developing feline visual cortex and cerebellum.
  • Assessment of antibody reactivity with phosphorylation-dependent and -independent epitopes.
  • Enzymatic treatment (alkaline phosphatase) to probe epitope accessibility.

    • Main Results:

    • MAbs exhibited differential staining patterns for MAP2 isoforms, influenced by location and developmental stage.
    • MAb AP18, targeting a phosphorylation-dependent epitope, showed persistent immunoreactivity in dendrites post-alkaline phosphatase treatment.
    • MAbs AP14, MT-01, and MT-02 recognized phosphorylation-independent epitopes in the central region of MAP2b.
    • Antibody MT-02 demonstrated dynamic staining of MAP2 in the cortex and cerebellum, changing with maturation and absent in adult cerebellar tissue.

    Conclusions:

    • Immunocytochemical detection of MAP2 is significantly influenced by molecular modifications like phosphorylation and conformational changes.
    • Different MAbs recognize distinct MAP2 epitopes, leading to varied staining outcomes.
    • These findings suggest that phosphorylation and specific conformations are key to MAP2 function and stability, and MAbs targeting these sites can elucidate MAP2's functional roles.