Immunomodulatory effects of Maharishi Amrit Kalash 4 and 5 in mice

To evaluate the immunomodulatory effects of two kinds of Ayurvedic food supplements (Maharishi Amrit Kalash 4 and Maharishi Amrit Kalash 5, M-4 and M-5), superoxide anion (O2-) production of peritoneal macrophages and the response of spleen cells to concanavalin A (Con A) were examined in mice given an aqueous emulsion of M-4 and M-5 p.o. at doses of 50 and 100 mg/kg for 10 days. O2- production of peritoneal macrophages in the M-5 (50 mg/kg)-treated group was significantly higher than that in the control group. The indices of stimulation of spleen cells by Con A were significantly (3 to 4 times) higher in groups treated with M-4 and M-5 at all doses than in the control group. These results indicate that M-4 enhances lymphocyte responsiveness and M-5 enhances not only lymphocyte responsiveness but also macrophage function. It is also suggested in this study that M-4 and M-5 have mitogenic effects on lymphocytes.