Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet activating factor modulates microvascular permeability through nitric oxide synthesis

M M Ramírez1, S M Quardt, D Kim

  • 1Department of Physiology, UMDNJ-New Jersey Medical School, Newark 07103-2714, USA.

Microvascular Research
|September 1, 1995
PubMed
Summary

Nitric oxide (NO) synthesis is integral to platelet-activating factor (PAF) signaling in microvessels. Inhibiting NO synthesis reduced PAF-induced vasoconstriction and microvascular permeability in hamsters.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimizing typhoid fever case definitions by combining serological tests in a large population study in Hechi City, China.

Epidemiology and infection·2007
Same author

Intra-arterial thrombolysis for acute stroke in patients 80 and older: a comparison of results in patients younger than 80 years.

AJNR. American journal of neuroradiology·2007
Same author

Influence of hepatitis C on renal function after liver transplantation.

Transplantation proceedings·2006
Same author

A comparison of twice-daily exenatide and biphasic insulin aspart in patients with type 2 diabetes who were suboptimally controlled with sulfonylurea and metformin: a non-inferiority study.

Diabetologia·2006
Same author

Hypermethylation of CpG island loci and hypomethylation of LINE-1 and Alu repeats in prostate adenocarcinoma and their relationship to clinicopathological features.

The Journal of pathology·2006
Same author

Endovascular mechanical clot retrieval in a broad ischemic stroke cohort.

AJNR. American journal of neuroradiology·2006

Area of Science:

  • Physiology
  • Pharmacology
  • Microcirculation Research

Background:

  • Biochemical signaling pathways regulate microvascular tone and permeability.
  • Platelet-activating factor (PAF) is a potent vasomediator.
  • The role of nitric oxide (NO) in PAF-mediated responses requires elucidation.

Purpose of the Study:

  • To test the hypothesis that NO synthesis is involved in the biochemical signaling pathway of PAF.
  • To investigate the effects of NO synthase (NOS) inhibition on microvascular responses to PAF.

Main Methods:

  • Utilized a hamster cheek pouch microvascular model.
  • Measured vessel diameter and FITC-dextran extravasation using intravital fluorescent microscopy and digital image analysis.
  • Employed NOS inhibitors N-Nitro-L-arginine methyl ester (L-NAME) and N-nitro-L-mono-methyl arginine (L-NMMA).

Related Experiment Videos

Main Results:

  • NOS inhibitors attenuated acetylcholine and bradykinin effects, indicating NOS activation.
  • PAF induced vasoconstriction and increased microvascular permeability.
  • Inhibition of NOS reduced both PAF-induced vasoconstriction and the increase in vascular permeability.

Conclusions:

  • NO synthesis is a crucial step in the postcapillary cellular response to PAF.
  • These findings support the involvement of NO in PAF's signaling pathway.
  • Understanding this pathway offers insights into microvascular regulation.