Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adhesion through L-selectin requires a threshold hydrodynamic shear

E B Finger1, K D Puri, R Alon

  • 1Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA.

Nature
|January 18, 1996
PubMed
Summary

A critical shear threshold is required for L-selectin-mediated cell rolling, unlike other selectins. This finding suggests a mechanism to prevent inappropriate leukocyte aggregation in low shear conditions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leukocyte rolling on engineered nanodot surfaces.

Micro & nano letters·2024
Same author

Opioid-prescribing patterns, storage, handling, and disposal in postoperative pediatric urology patients.

Journal of pediatric urology·2019
Same author

Molecular Characterization of Fusarium Head Blight Pathogens Sampled from a Naturally Infected Disease Nursery Used for Wheat Breeding Programs in China.

Plant disease·2019
Same author

Virulence Characterization of International Collections of the Wheat Stripe Rust Pathogen, Puccinia striiformis f. sp. tritici.

Plant disease·2019
Same author

Measurement of the centrality dependence of the charged-particle pseudorapidity distribution in proton-lead collisions at [Formula: see text] TeV with the ATLAS detector.

The European physical journal. C, Particles and fields·2017
Same author

Defining the Tipping Point in Surgical Performance for Laparoscopic Donor Nephrectomy Among Transplant Surgery Fellows: A Risk-Adjusted Cumulative Summation Learning Curve Analysis.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016

Area of Science:

  • Immunology
  • Biophysics
  • Cell Biology

Background:

  • Selectins are cell adhesion molecules crucial for leukocyte trafficking in vascular shear flow.
  • Selectin-ligand bonds exhibit high mechanical strength and fast kinetics, enabling leukocyte tethering and rolling.
  • L-selectin mediates leukocyte interactions with lymph node addressins and other leukocytes.

Purpose of the Study:

  • To investigate the role of shear stress in regulating selectin-mediated cell adhesion.
  • To identify unique mechanisms governing L-selectin interactions compared to other selectins.
  • To understand the physiological implications of shear-dependent L-selectin function.

Main Methods:

  • In vitro studies of cell adhesion under controlled shear flow.
  • In vivo experiments to observe leukocyte-endothelial interactions.

Related Experiment Videos

  • Comparative analysis of L-selectin, E-selectin, P-selectin, and VCAM-1 under varying shear conditions.

    • Main Results:

    • L-selectin-mediated rolling requires shear stress above a critical threshold.
    • E-selectin, P-selectin, and VCAM-1 mediated rolling do not exhibit this shear threshold.
    • This shear-dependent regulation was observed in both in vitro and in vivo settings.

    Conclusions:

    • A shear threshold mechanism uniquely regulates L-selectin-mediated leukocyte rolling.
    • This finding provides insight into preventing unwanted leukocyte aggregation in low shear environments.
    • The study reveals a novel regulatory pathway for leukocyte adhesion and trafficking.