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Functional evidence for indirect recognition of G.U in tRNA(Ala) by alanyl-tRNA synthetase
K Gabriel1, J Schneider, W H McClain
1Department of Bacteriology, University of Wisconsin, Madison 53706, USA.
Science (New York, N.Y.)
|January 12, 1996
Abstract:
The structural features of the G.U wobble pair in Escherichia coli alanine transfer RNA (tRNA(Ala)) that are associated with aminoacylation by alanyl-tRNA synthetase (AlaRS) were investigated in vivo for wild-type tRNA(Ala) and mutant tRNAs with G.U substitutions. tRNA(Ala) with G.U, C.A, or G.A gave similar amounts of charged tRNA(Ala) and supported viability of E. coli lacking chromosomal tRNA(Ala) genes. tRNA(Ala) with G.C was inactive. Recognition of G.U by AlaRS thus requires more than the functional groups on G.U in a regular helix and may involve detection of a helical distortion.