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Updated: Oct 13, 2026

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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
Published on: February 23, 2021
Sequence and analysis of the rpoB gene of Mycobacterium smegmatis
S V Hetherington1, A S Watson, C C Patrick
1Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Antimicrobial Agents and Chemotherapy
|September 1, 1995
Abstract:
The rpoB gene encodes the beta subunit of the DNA-dependent RNA polymerase of bacteria. Mutations in defined areas result in resistance to rifampin. Mycobacterium smegmatis is naturally resistant to rifampin, but analysis of the rpoB gene revealed no identifiable rifampin resistance mutations. Another mechanism of resistance may be present.

