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Human excimer laser keratectomy. Immunohistochemical analysis of healing
J A Anderson1, P S Binder, M E Rock
1National Vision Research Institute, San Diego, Calif, USA.
Objective:
To analyze human corneal responses to excimer laser by immunohistochemistry.
Methods:
Corneas cultured for 3 weeks after laser ablation or taken from patients 5 to 16 months after laser treatment were exposed to antibodies to beta, integrin; types VII, IV, and III collagen; fibronectin; type I procollagen; and prolyl 4-hydroxylase. Antibody distributions were compared with those of normal corneas and unablated regions of treated corneas.
Results:
After 3 weeks, distribution of beta, integrin, and types VII and IV collagen was patchy; heavy deposits of fibronectin appeared subepithelially. Keratocytes stained for prolyl-4-hydroxylase and type I procollagen. With increasing postoperative time, fibronectin diminished, and beta 4 integrin and type VII collagen became linear, stromal staining of types III and IV collagen increased and their diminished.
Conclusions:
Components of basement membrane, attachment complexes, and stromal matrix are synthesized shortly after laser treatment. Changes in these elements persist for 16 months in the human cornea.