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Alteration by EGTA of the human red cell Ca(2+)-ATPase
1Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela.
Biochimica Et Biophysica Acta
|December 13, 1995
Abstract:
The effect of EGTA on Ca(2+)-ATPase activity was studied in fragmented membranes and solubilized preparations from human red cells. A dual action was found. At low concentrations (0.1-1 mM), EGTA increased Ca2+ affinity without affecting Vmax. By contrast, at high concentrations (5-10 mM), EGTA was inhibitory. Both effects were partially reversible.