Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Uridine, a cell division factor in pea roots

G Smit1, C C de Koster, J Schripsema

  • 1Institute of Molecular Plant Sciences, Leiden University, The Netherlands.

Plant Molecular Biology
|November 1, 1995
PubMed
Summary

Researchers found uridine, a naturally occurring molecule, in pea roots. This compound enhances hormone-induced cell proliferation, potentially acting as a morphogen in root nodule formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Symmetric expression of ohnologs encoding conserved antiviral responses in tetraploid common carp suggest absence of subgenome dominance after whole genome duplication.

Genomics·2023
Same author

A full-body transcriptome and proteome resource for the European common carp.

BMC genomics·2016
Same author

Transcriptomic Approaches in the Zebrafish Model for Tuberculosis-Insights Into Host- and Pathogen-specific Determinants of the Innate Immune Response.

Advances in genetics·2016
Same author

Imaging Cancer Angiogenesis and Metastasis in a Zebrafish Embryo Model.

Advances in experimental medicine and biology·2016
Same author

Exploring the potential of Burkholderia sacchari to produce polyhydroxyalkanoates.

Journal of applied microbiology·2013
Same author

Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI.

Plant molecular biology·2013

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • Legume root nodule formation is a complex process initiated by cell division and primordia development in the root cortex.
  • This process typically occurs at specific sites, often aligned with protoxylem ridges in the central root cylinder.

Purpose of the Study:

  • To identify factors within pea roots that influence hormone-induced cell proliferation in root cortex explants.
  • To investigate the role of these factors in the spatial positioning of root nodule primordia.

Main Methods:

  • Isolation of a growth-promoting factor from the central cylinder (stele) of pea roots.
  • Testing the effect of the isolated factor on hormone-induced cell proliferation in root cortex explants.
  • Identification of the isolated factor using biochemical and analytical techniques.

Related Experiment Videos

Main Results:

  • A factor enhancing hormone-induced cell proliferation was successfully isolated from pea root stele.
  • The isolated factor was identified as uridine.
  • Uridine demonstrated efficacy at picomolar concentrations, suggesting a significant biological role.

Conclusions:

  • Uridine is present in pea roots and can stimulate cell proliferation in root cortex explants.
  • Uridine may function as a morphogen, guiding the precise location of root nodule primordia formation.
  • This finding offers new insights into the molecular mechanisms regulating legume symbiosis and root development.