Complement activation by angiographic catheters in vitro

Four different polymers used in commercial angiographic catheters were compared in vitro with respect to their ability to activate the complement system.

Commercially available angiographic catheters made from one of the following plastics were used: polyamide, polyethylene, polyurethane, and polytetrafluoroethylene. Silicone-coated latex urinary catheters served as the reference standard. Each catheter was cut into 20-mm segments, immersed in a polypropylene tube containing fresh serum from a volunteer donor, and incubated at 37 degrees C. Samples were drawn at 15 minutes, 1 hour, and 6 hours; C3 activation products (C3AP) and the terminal complement complex (TCC) content were estimated with enzyme immunoassays.

By 1 hour, a significant increase in C3AP and TCC concentrations was observed with all angiographic catheters relative to controls (P < .01-.001). The time-concentration plots for both C3AP and TCC were steepest for polyamide. C3AP concentrations relative to controls were significantly higher with exposure to polyamide compared with polyurethane at 1 hour (P < .01), and with both polyethylene and polyurethane at 6 hours (P < .01). Polytetrafluoroethylene induced larger amounts of C3AP formation by 6 hours than polyethylene and polyurethane (P < .05). However, polytetrafluoroethylene was associated with the lowest relative median concentrations of TCC; the difference with polyamide was significant at 6 hours (P < .001). As with C3AP, differences in TCC generation between polyethylene and polyurethane were marginal at all observation points (P > .05).

All the polymers tested activated the complement system. Activation was most prominent with exposure to polyamide and least marked with polyurethane.