Related Experiment Video
Updated: Aug 15, 2026

Glycopeptide Capture for Cell Surface Proteomics
Published on: May 9, 2014
Biochemical approaches to the detection and characterization of membrane proteins carrying blood group determinants
1Centre Régional de Transfusion Sanguine, Nantes.
Abstract:
The investigation of red blood cell membrane proteins carrying blood group determinants mainly involves the use of specific antibodies--polyclonal human antibodies and both murine and human monoclonal antibodies--directed against blood group antigens. Other blood group specific reagents like lectins, also represent useful tools to identify membrane proteins. These reagents allowed the detection and, then the characterization of several red cell membrane components by using a series of methods based on their specific interaction with the corresponding antigen. Reagents and investigation methods are overviewed hereafter.
Related Concept Videos
Membrane Carbohydrates
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

