Duffy and receptors for P. vivax and chemotactic peptides

The Duffy blood group system consists of two principal antigens, Fya and Fyb produced by FY*A and FY*B co-dominant alleles. Antisera, anti-Fya and anti-Fyb, define four phenotypes: Fy(a+b-), Fy(a-b+), Fy(a+b+) and Fy(a-b-). Neither antiserum agglutinates Fy(a-b-) cells, the predominant phenotype in Blacks. Outside the Black population, Fy(a-b-) phenotype is very rare. Duffy antigens appear to be multimeric erythrocyte-membrane proteins composed of different subunits. A glycoprotein of 35-45 kDa, gp-Fy, is the major subunit of the complex and has antigenic determinants defined by Duffy antibodies. The protein consists of 337 amino acid residues with a M(r) of 35,733, the same as deglycosylated gp-Fy. The hydropathy map predicts an exocellular N-terminal domain of 64 residues, nine transmembrane alpha-helices, three short protruding hydrophilic loops and an endocellular C-terminal domain of 23 residues. Duffy specific transcript, a approximately 1.3 kb mRNA, is produced by the bone marrow of Duffy-positive individuals, but it is not produced by the bone marrow of Duffy-negative individuals. The same size mRNA is produced in many tissues of Duffy-positive individuals. The same tissues of Duffy-negative individuals also synthesize the same size mRNA and the same gp-Fy as that of Duffy-positive individuals. There is not, therefore, Duffy null phenotype in the Black population. The difference between FY*A and FY*B alleles is a single nucleotide change at position 306; guanine is in FY*A, and adenine is in FY*B.(ABSTRACT TRUNCATED AT 250 WORDS)