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Urea transport and Kidd blood groups

J P Cartron1, P Ripoche

  • 1INSERM U76, Institut National de la Transfusion Sanguine, Paris.

Transfusion Clinique Et Biologique : Journal De La Societe Francaise De Transfusion Sanguine
|January 1, 1995
PubMed
Summary

The Kidd blood group gene locus encodes the human erythrocyte urea transporter (HUT11). This discovery links the Kidd protein to urea transport defects in Jk(a-b-) individuals, impacting red blood cell function.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Physiology

Background:

  • The human erythrocyte urea transporter (HUT11) gene was recently cloned.
  • The HUT11 gene is localized to chromosome 18q12-q21, similar to the Kidd blood group gene.
  • Individuals with the Jk(a-b-) phenotype exhibit a selective defect in urea transport.

Purpose of the Study:

  • To investigate the molecular identity of the human erythrocyte urea transporter.
  • To determine if the Kidd blood group locus is associated with urea transport in red blood cells.

Main Methods:

  • In vitro translation of HUT11 cDNA.
  • Immunoprecipitation using anti-Jk3 antibody.
  • Western blot analysis with an anti-HUT11 N-terminal antibody.
  • N-glycanase digestion of erythrocyte proteins.

Main Results:

  • HUT11 cDNA directed the synthesis of a 36 kDa protein immunoprecipitated by anti-Jk3 antibody.
  • Anti-Jk3 and anti-HUT11 antibodies detected a 46-60 kDa glycoprotein in red blood cells, absent in Jk(a-b-) individuals.
  • N-glycanase digestion reduced the glycoprotein size to 36 kDa, consistent with the in vitro translation product.
  • Jk(a-b-) red blood cells lack both Kidd protein and HUT11 urea transporter, showing normal water transport and aquaporin-1 expression.

Conclusions:

  • The Kidd locus encodes the human erythrocyte urea transporter (HUT11).
  • This finding establishes a direct link between the Kidd blood group and urea transport function.
  • Opens new avenues for studying urea transporter biology and tissue-specific regulation.

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