Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Re-creating the RNA world

I Hirao1, A D Ellington

  • 1Department of Chemistry, Indiana University, Bloomington 47405, USA.

Current Biology : CB
|September 1, 1995
PubMed
Summary

In vitro selection experiments model the RNA world

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into proteins" [J. Biotechnol. 178 (2014) 12-22].

Journal of biotechnology·2016
Same author

A stochastic DNA walker that traverses a microparticle surface.

Nature nanotechnology·2015
Same author

Using RecA protein to enhance kinetic rates of DNA circuits.

Chemical communications (Cambridge, England)·2015
Same author

Design, synthesis, and amplification of DNA pools for in vitro selection.

Current protocols in nucleic acid chemistry·2008
Same author

In vitro selection of RNA aptamers to a protein target by filter immobilization.

Current protocols in nucleic acid chemistry·2008
Same author

Design, synthesis, and amplification of DNA pools for construction of combinatorial pools and libraries.

Current protocols in molecular biology·2008

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Origin of Life Studies

Background:

  • The RNA world hypothesis proposes that RNA was the primary form of genetic material and catalytic molecule.
  • In vitro selection is a powerful technique for evolving RNA molecules with specific functions.

Purpose of the Study:

  • To construct and test models for the evolution of the RNA world using in vitro selection data.
  • To investigate the challenges in identifying molecular fossils from the RNA world.

Main Methods:

  • In vitro selection experiments were performed to isolate functional RNA molecules.
  • Computational modeling was used to simulate RNA evolution.

Main Results:

  • Selected RNAs demonstrated significant ligand-binding and catalytic capabilities.
  • The high success of selected RNAs complicates the precise lineage determination of molecular fossils.

Conclusions:

  • While in vitro selection aids RNA world modeling, it presents challenges for identifying ancient RNA lineages.
  • Further research is needed to refine methods for tracing molecular fossils from the early RNA era.

Related Experiment Videos