Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein folding. Prolyl isomerases join the fold

F X Schmid1

  • 1Biochemisches Laboratorium, Universität Bayreuth, Germany.

Current Biology : CB
|September 1, 1995
PubMed
Summary

Cyclophilins, enzymes with prolyl isomerase activity, are now shown to speed up the folding of mitochondrial precursor proteins, providing cellular evidence for their role in protein folding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitral prolapse caused by lymphangioma.

The Thoracic and cardiovascular surgeon·2005
Same author

Adequacy of perfusion during hypothermia: regional distribution of cardiopulmonary bypass flow, mixed venous and regional venous oxygen saturation - hypothermia and distribution of flow and oxygen.

The Thoracic and cardiovascular surgeon·2003
Same author

[Extracorporeal lung support and endovascular stent in traumatic aortic rupture and severe lung failure].

Der Unfallchirurg·2002
Same author

Pumpless extracorporeal lung assist using an arterio-venous shunt. Applications and limitations.

Minerva anestesiologica·2002
Same author

Prolyl isomerases.

Advances in protein chemistry·2002
Same author

Electrostatic stabilization of a thermophilic cold shock protein.

Journal of molecular biology·2002

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Cyclophilins are known enzymes possessing prolyl isomerase activity.
  • Their proposed function in cellular protein folding has lacked substantial evidence.

Purpose of the Study:

  • To investigate the role of cyclophilins in the folding of mitochondrial precursor proteins.
  • To provide evidence for cyclophilin involvement in cellular protein folding processes.

Main Methods:

  • Enzyme assays measuring prolyl isomerase activity.
  • Studies on the folding kinetics of mitochondrial precursor proteins in vitro and in cellulo.

Main Results:

  • Cyclophilins were found to significantly accelerate the folding of mitochondrial precursor proteins.
  • This acceleration demonstrates a direct role for cyclophilins in facilitating protein maturation.

Conclusions:

  • Cyclophilins play a crucial role in promoting the correct folding of mitochondrial proteins.
  • These findings provide strong evidence for the function of cyclophilins as cellular protein folding chaperones.

Related Experiment Videos